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The "dilution factor" is an expression which describes the ratio of the aliquot volume to the final volume. Dilution factor is a notation often used in commercial assays. For example, in solution with a 1/5 dilution factor (which may be abbreviated as x5 dilution ), entails combining 1 unit volume of solute (the material to be diluted) with ...
The volume of the squares counted is the one shown in the table at the top, depending on the size (see figure on the right). The number of cells counted is the sum of all cells counted across squares in one chamber. The proportion of the cells counted applies if not all inner squares within a set square are counted (i.e., if only 4 out of the ...
Three plates are needed for each dilution series, for statistical reasons an average of at least 3 counts are needed. The surface of the plates need to be sufficiently dry to allow a 20μl drop to be absorbed in 15–20 minutes. Plates are divided into equal sectors (it is possible to use up to 8 per plate).
Conversely, in an indirect dilution assay the dose levels are administered at fixed dose levels, so that the response is a stochastic variable. In some assays, there may be strong reasons for believing that all the constituents of the test preparation except one, are without any effect on the studied response of the subjects.
A prior understanding of the microscopic anatomy of the organism can give a better understanding of how the observed CFU/mL relates to the number of viable cells per milliliter. Alternatively it is possible to decrease the average number of cells per CFU in some cases by vortexing the sample before conducting the dilution. However, many ...
3. Medications. Some medications have been associated with temporary hair loss. Most of the time hair loss related to medication is due to the drug disrupting the hair growth cycle leading to a ...
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A serial dilution is the step-wise dilution of a substance in solution, either by using a constant dilution factor, or by using a variable factor between dilutions. If the dilution factor at each step is constant, this results in a geometric progression of the concentration in a logarithmic fashion.