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This is the immersion of plant material directly in liquid nitrogen, or after desiccation. This is often done with (orthodox) seeds that already have a low moisture content or pollen. [8] This method cannot be used with tissues that contain a lot of water or are sensitive to dehydration. Slow freezing. This method relies on the mechanism of ...
Typically, a sample is plunged into liquid nitrogen or into liquid ethane or liquid propane in a container cooled by liquid nitrogen. The ultimate objective is to freeze the specimen so rapidly (at 10 4 to 10 6 K per second) that ice crystals are unable to form, or are prevented from growing big enough to cause damage to the specimen's ...
A tank of liquid nitrogen, used to supply a cryogenic freezer (for storing laboratory samples at a temperature of about −150 °C or −238 °F) Controlled-rate and slow freezing, also known as slow programmable freezing (SPF), [18] is a technique where cells are cooled to around -196 °C over the course of several hours.
A cryoprotectant is a substance used to protect biological tissue from freezing damage (i.e. that due to ice formation). Arctic and Antarctic insects, fish and amphibians create cryoprotectants (antifreeze compounds and antifreeze proteins) in their bodies to minimize freezing damage during cold winter periods. Cryoprotectants are also used to ...
Liquid nitrogen and similar cryosurgery methods are common surgical treatments, which act by freezing the external cell structure of the warts, destroying the live tissue. [citation needed] Electrodesiccation and surgical excision may produce scarring. [citation needed]
Cryogenic IQF freezers immerse the product in liquid nitrogen at very low temperatures, freezing it rapidly while continuously moving the product to avoid block or lump formation. [5] Although this method shows good freezing results, it might lead to higher processing costs per weight of product due to the cost of the liquid nitrogen required.
In vacuum applications, a cold trap is a device that condenses all vapors except the permanent gases (hydrogen, oxygen, and nitrogen) into a liquid or solid. [ 2 ] [ needs update ] The most common objective is to prevent vapors being evacuated from an experiment from entering a vacuum pump where they would condense and contaminate it.
Since then, machines that freeze biological samples using programmable steps, or controlled rates, have been used all over the world for human, animal, and cell biology – 'freezing down' a sample to better preserve it for eventual thawing, before it is deep frozen, or cryopreserved, in liquid nitrogen. Such machines are used for freezing ...
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