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The inflection point at which the increase in response with increasing ligand concentration begins to slow is the EC 50, which can be mathematically determined by derivation of the best-fit line. While relying on a graph for estimation is more convenient, this typical method yields less accurate and precise results.
The parameters of the dose response curve reflect measures of potency (such as EC50, IC50, ED50, etc.) and measures of efficacy (such as tissue, cell or population response). A commonly used dose–response curve is the EC 50 curve, the half maximal effective concentration, where the EC 50 point is defined as the inflection point of the curve.
EC50 is incorporated in the Hill's Equation, a function that demonstrates the relationship between agonist concentration and ligand binding. EC50 is mathematically given as the inflection point of the equation. [33]
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The relationship between and EC50 may be quite complex as a biological response will be the sum of myriad factors; a drug will have a different biological effect if more receptors are present, regardless of its affinity.
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