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N-(1-Naphthyl)ethylenediamine dihydrochloride is widely used in the quantitative analysis of nitrate and nitrite in water samples by colorimetry.It readily undergoes a diazonium coupling reaction in the presence of nitrite to give a strongly colored azo compound.
Prepare 800 mL of distilled water in a suitable container. Add 8 g of NaCl to the solution. Add 400 mg of KCl to the solution. Add 140 mg of CaCl 2 to the solution. Add 100 mg of MgSO 4-7H 2 O to the solution. Add 100 mg of MgCl 2-6H 2 O to the solution. Add 60 mg of Na 2 HPO 4-2H 2 O to the solution. Add 60 mg of KH 2 PO 4 to the solution.
Lysis buffer usually contains one or more salts. The function of salts in lysis buffer is to establish an ionic strength in the buffer solution. Some of the most commonly used salts are NaCl, KCl, and (NH 4) 2 SO 4. They are usually used with a concentration between 50 and 150 mM. [4] Sodium dodecyl sulfate (SDS) structure
Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range. The conjugate acid of Tris has a pK a of 8.07 at 25 °C. The pK a declines approximately 0.03 units per degree Celsius rise in temperature. This can lead to relatively dramatic pH shifts when there are shifts in solution temperature.
Ethacridine is also used as an agent for second trimester abortion. Up to 150 ml of a 0.1% solution is instilled extra-amniotically using a foley catheter. After 20 to 40 hours, 'mini labor' ensues. In China, an intra-amniotic method has also been used. [3]
To make a 100 ml solution of T 10 E 1 buffer, 1 ml of 1 M Tris base (pH 10–11) and 0.2 ml EDTA (0.5 M) are mixed and made up with double distilled water up to 100ml. Add microliter amounts of high molarity HCl to lower the pH to 8. Based on nuclease studies from the 1980s, the pH is usually adjusted to 7.5 for RNA and 8.0 for DNA.
The pH of a sodium chloride solution remains ≈7 due to the extremely weak basicity of the Cl − ion, which is the conjugate base of the strong acid HCl. In other words, NaCl has no effect on system pH [32] in diluted solutions where the effects of ionic strength and activity coefficients are negligible.
Cypermethrin is very toxic to cats which cannot tolerate the therapeutic doses for dogs. [6] This is associated with UGT1A6 deficiency in cats, the enzyme responsible for metabolizing cypermethrin. As a consequence, cypermethrin remains much longer in the cat's organs than in dogs or other mammals and can be fatal in large doses.
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