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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
If you have data from a spreadsheet document (e.g. LibreOffice Calc) or in a statistics software R/R-Studio, you can export them to CSV file. The CSV file can be loaded with an v:en:AppLSAC, that is able to convert the CSV in chart for the data. The column should have headers in the first row.
English: Gray, blue, red, green, light green, black graph papers with 1 cm–0.5 cm–1 mm grids (page size: A4) in printable PDF format. Date 25 July 2013, 18:04:17
Kingsoft Office Spreadsheets 2012 – For MS Windows. Both free and paid versions are available. It can handle Microsoft Excel .xls and .xlsx files, and also produce other file formats such as .et, .txt, .csv, .pdf, and .dbf. It supports multiple tabs, VBA macro and PDF converting. [10] Lotus SmartSuite Lotus 123 – for MS Windows. In its MS ...
Pyrosequencing, a new method of DNA sequencing which involves the addition of phosphorylated dNTP's to the DNA polymerase reaction which emmit light upon binding to the DNA template. It makes DNA sequencing significantly faster than with the current chain termination method. We do use them, look at Pyrosequencing/Temp.
Pyrosequencing has also been used to analyze bisulfite-treated DNA without using methylation-specific PCR. [7] [8] Following PCR amplification of the region of interest, pyrosequencing is used to determine the bisulfite-converted sequence of specific CpG sites in the region. The ratio of C-to-T at individual sites can be determined ...
Numbers uses a free-form "canvas" approach that demotes tables to one of many different media types placed on a page. Other media, like charts , graphics , and text, are treated as peers. In comparison, traditional spreadsheets like Microsoft Excel use the table as the primary container, with other media placed within the table.