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The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
Chocolate agar showing Francisella tularensis colonies Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, the nonselective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other than those of N. gonorrhoeae, while the selective Thayer–Martin medium on the right, containing ...
The phage can then be isolated from the resulting plaques in a lawn of bacteria on a plate. Viral cultures are obtained from their appropriate eukaryotic host cells. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar ...
The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered. The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern.
The sample is then cooled and inspected for pour point as per the usual pour point method. The method usually gives higher pour point because the thermal history has not been cancelled by a prolonged thermal treatment. The lower pour point is measured by first pouring the sample into a stainless steel pressure vessel. The vessel is then screwed ...
In Lippmann's method, a glass plate is coated with an ultra fine grain [6] light-sensitive film (originally using the albumen process containing potassium bromide; later and primarily using silver halide gelatin), [7] then dried, sensitized in the silver bath, washed, irrigated with cyanine solution, and dried again. The back of the film is ...
Before How to Win Friends and Influence People was released, the genre of self-help books had an ample heritage. [citation needed] Authors such as Orison Swett Marden and Samuel Smiles had enormous success with their self-help books in the late 19th and early 20th centuries.
Discourse on the Method of Rightly Conducting One's Reason and of Seeking Truth in the Sciences (French: Discours de la Méthode pour bien conduire sa raison, et chercher la vérité dans les sciences) is a philosophical and autobiographical treatise published by René Descartes in 1637.