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Retention distance, or R D, is a concept in thin layer chromatography, designed for quantitative measurement of equal-spreading of the spots on the chromatographic ...
In gas chromatography, the Kovats retention index (shorter Kovats index, retention index; plural retention indices) is used to convert retention times into system-independent constants. The index is named after the Hungarian-born Swiss chemist Ervin Kováts , who outlined the concept in the 1950s while performing research into the composition ...
The column used for GPC is filled with a microporous packing material. The column is filled with the gel. Since the total penetration volume is the maximum volume permeated by the analytes, and there is no retention on the surface of the stationary phase, the total column volume is usually large, relatively to the sample volume.
Example chromatogram showing signal as a function of retention time. In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1] [2] [3] [4]
The linear isotherm occurs when the solute concentration needed to be purified is very small relative to the binding molecule. Thus, the equilibrium can be defined as: [CS] = K eq [C]. For industrial scale uses, the total binding molecules on the column resin beads must be factored in because unoccupied sites must be taken into account.
The void volume includes any particles too large to enter the medium, and the solvent volume is known as the column volume. Following are the materials which are commonly used for porous gel beads in size exclusion chromatography [ 20 ]
In chromatography, the retardation factor (R) is the fraction of an analyte in the mobile phase of a chromatographic system. [1] In planar chromatography in particular, the retardation factor R F is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front. [2]
The helium gas may now go from the reference chamber to the sample chamber thanks to the connection between the two rooms. The volume of the sample solid is calculated using the ratio between the starting and final pressures. The pore volume, as calculated by the helium pycnometer, is the difference between the total volume and the solid volume ...