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Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens .
During cell migration, both the composition and the morphology of the focal adhesion change. Initially, small (0.25μm 2) focal adhesions called focal complexes (FXs) are formed at the leading edge of the cell in lamellipodia: they consist of integrin, and some of the adapter proteins, such as talin, paxillin and tensin. Many of these focal ...
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope. It is a valuable tool for the determination of cellular contents from individual cells.
Immunofluorescence image of HeLa cells grown in tissue culture and stained with antibody to actin in green, vimentin in red and DNA in blue Immunofluorescence of HeLa cells showing microtubules in green, mitochondria in yellow, nucleoli in red and nuclear DNA in purple. HeLa (/ ˈ h iː l ɑː /) is an immortalized cell line used in scientific ...
Schematic of cell adhesion. Cell adhesion is the process by which cells interact and attach to neighbouring cells through specialised molecules of the cell surface. This process can occur either through direct contact between cell surfaces such as cell junctions or indirect interaction, where cells attach to surrounding extracellular matrix, a gel-like structure containing molecules released ...
Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur. [1] Most vertebrate derived cells (with the exception of hematopoietic cells) can be cultured and require a 2 dimensional monolayer that to facilitate cell adhesion and spreading. [2]
Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.
Cells are immunostained in solution using methods similar to those used for immunofluorescence, and then analysed by flow cytometry. [ citation needed ] Flow cytometry has several advantages over IHC including: the ability to define distinct cell populations by their size and granularity; the capacity to gate out dead cells; improved ...