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  2. Sequencing - Wikipedia

    en.wikipedia.org/wiki/Sequencing

    DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates.

  3. List of sequenced eukaryotic genomes - Wikipedia

    en.wikipedia.org/wiki/List_of_sequenced...

    Saccharomyces cerevisiae was the first eukaryotic organism to have its complete genome sequence determined.. This list of "sequenced" eukaryotic genomes contains all the eukaryotes known to have publicly available complete nuclear and organelle genome sequences that have been sequenced, assembled, annotated and published; draft genomes are not included, nor are organelle-only sequences.

  4. DNA sequencer - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencer

    SOLiD applies sequencing by ligation and dual base encoding. The first SOLiD system was launched in 2007, generating reading lengths of 35bp and 3G data per run. After five upgrades, the 5500xl sequencing system was released in 2010, considerably increasing read length to 85bp, improving accuracy up to 99.99% and producing 30G per 7-day run. [10]

  5. Sequence diagram - Wikipedia

    en.wikipedia.org/wiki/Sequence_diagram

    A system sequence diagram should be done for the main success scenario of the use case, and frequent or complex alternative scenarios. There are two kinds of sequence diagrams: Sequence Diagram (SD): A regular version of sequence diagram describes how the system operates, and every object within a system is described specifically.

  6. DNA sequencing - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencing

    The first of the high-throughput sequencing technologies, massively parallel signature sequencing (or MPSS, also called next generation sequencing), was developed in the 1990s at Lynx Therapeutics, a company founded in 1992 by Sydney Brenner and Sam Eletr. MPSS was a bead-based method that used a complex approach of adapter ligation followed by ...

  7. Whole genome sequencing - Wikipedia

    en.wikipedia.org/wiki/Whole_genome_sequencing

    The first published description of the use of paired ends was in 1990 as part of the sequencing of the human HPRT locus, [29] although the use of paired ends was limited to closing gaps after the application of a traditional shotgun sequencing approach. The first theoretical description of a pure pairwise end sequencing strategy, assuming ...

  8. DNA sequencing theory - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencing_theory

    DNA sequencing theory is the broad body of work that attempts to lay analytical foundations for determining the order of specific nucleotides in a sequence of DNA, otherwise known as DNA sequencing. The practical aspects revolve around designing and optimizing sequencing projects (known as "strategic genomics"), predicting project performance ...

  9. Sequence assembly - Wikipedia

    en.wikipedia.org/wiki/Sequence_assembly

    In bioinformatics, sequence assembly refers to aligning and merging fragments from a longer DNA sequence in order to reconstruct the original sequence. [1] This is needed as DNA sequencing technology might not be able to 'read' whole genomes in one go, but rather reads small pieces of between 20 and 30,000 bases, depending on the technology used. [1]

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