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snRNA-seq uses isolated nuclei instead of the entire cells to profile gene expression. That is to say, scRNA-seq measures both cytoplasmic and nuclear transcripts, while snRNA-seq mainly measures nuclear transcripts (though some transcripts might be attached to the rough endoplasmic reticulum and partially preserved in nuclear preps). [7]
Combining FACS with scRNA-seq has produced optimized protocols such as SORT-seq. [8] A list of studies that utilized SORT-seq can be found here. [9] Moreover, combining microfluidic devices with scRNA-seq has been optimized in 10x Genomics protocols. [10]
Like typical next-generation sequencing experiments, single-cell sequencing protocols generally contain the following steps: isolation of a single cell, nucleic acid extraction and amplification, sequencing library preparation, sequencing, and bioinformatic data analysis. It is more challenging to perform single-cell sequencing than sequencing ...
Protocols for Recombinant DNA Isolation, Cloning, and Sequencing This page was last edited on 20 July 2024, at 20:42 (UTC). Text is available under the Creative ...
10x Genomics was founded in 2012 by Serge Saxonov, Ben Hindson and Kevin Ness to create advanced testing equipment for use in cellular biology. [3] Prior to starting the company, Saxonov was the founding architect, and director of research and development at 23andMe. [2]
A nuclear run-on assay is conducted to identify the genes that are being transcribed at a certain time point. Approximately one million cell nuclei are isolated and incubated with labeled nucleotides, and genes in the process of being transcribed are detected by hybridization of extracted RNA to gene specific probes on a blot. [1]
The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue.
VINE-seq [1] (Vessel Isolation and Nuclei Extraction for Sequencing) is a method to isolate and molecularly characterize the vascular and perivascular cells of the human brain microvessels at single-nuclei resolution.