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Thus a supravital stain may have a greater toxicity, as only a few cells need to survive it a short while. The term "vital stain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. As the cells are alive ...
In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain , the core concept being that the cell ...
For example, tissues that stain with an azure stain may be referred to as azurophilic. This may also be used for more generalized staining properties, such as acidophilic for tissues that stain by acidic stains (most notably eosin ), basophilic when staining in basic dyes, and amphophilic [ 25 ] when staining with either acid or basic dyes.
The term "vital stain" is occasionally used interchangeably with both intravital and supravital stains, the underlying concept in either case being that the cells examined are still alive. In a stricter sense, the term "vital staining" means the polar opposite of "supravital staining."
Polychromasia is a disorder where there is an abnormally high number of immature red blood cells found in the bloodstream as a result of being prematurely released from the bone marrow during blood formation (poly- refers to many, and -chromasia means color.)
Brilliant cresyl blue is a supravital stain used for counting reticulocytes. [2] It is classified as an oxazine dye. N95 dust masks, eye shields, and gloves must all be worn when handling the chemical. [3]
It is a supravital stain. [2] It is closely related to methylene blue, an older stain in wide use. Safety. New methylene blue is toxic. Skin contact or inhalation ...
Heinz body stain of feline blood, showing three distinct Heinz bodies. Heinz bodies appear as small round inclusions within the red cell body, though they are not visible when stained with Romanowsky dyes. They are visualized more clearly with supravital staining [5] [6] (e.g., with new methylene blue, crystal violet or bromocresol green).