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2. Dilution cloning - Wikipedia

   en.wikipedia.org/wiki/Dilution_cloning

   Appropriate dilutions are then made, depending on cell number in the starting population, as well as the viability and characteristics of the cells being cloned. After the final dilutions are produced, aliquots of the suspension are plated [1] or placed in wells [2] and incubated. If all works correctly, a monoclonal cell colony will be produced.

3. 3D cell culture - Wikipedia

   en.wikipedia.org/wiki/3D_cell_culture

   One common method is to use low cell adhesion plates, typically a 96 well plate, to mass-produce spheroid cultures, where the aggregates form in the rounded bottom of the cell plates. [36] [42] Spheroids can also be cultured using the hanging drop method [43] involving forming cell aggregates in drops that hang from the surface of a cell plate ...

4. Microplate - Wikipedia

   en.wikipedia.org/wiki/Microplate

   The well position is also standardized, but only for 96- , 384-, and 1536-well plates. These are generally well followed by manufacturers: Well Positions [16] [17] 96-well plates have a 9 mm well-to-well spacing, 384-wells a 4.5 mm spacing, and 1536-wells a 2.25 mm spacing. A notable characteristic is that the well array is symmetrical when the ...

5. Cell sorting - Wikipedia

   en.wikipedia.org/wiki/Cell_sorting

   Methods of cell sorting fall into two major categories: fluorescence-activated cell sorting (FACS) and immunomagnetic cell sorting. [2] Due to many years of refinement and increased demand for cell separation however, researchers are working to develop microfluidic sorting devices that have many benefits in comparison to the main types of fluorescence-activated cell sorting and immunomagnetic ...

6. Liquid scintillation counting - Wikipedia

   en.wikipedia.org/wiki/Liquid_scintillation_counting

   Newer machines may use 96-well plates with individual filters in each well. Many counters have two photo multiplier tubes connected in a coincidence circuit . The coincidence circuit assures that genuine light pulses, which reach both photomultiplier tubes, are counted, while spurious pulses (due to line noise , for example), which would only ...

7. ELISA - Wikipedia

   en.wikipedia.org/wiki/ELISA

   A buffered solution of the antigen to be tested for is added to each well (usually 96-well plates) of a microtiter plate, where it is given time to adhere to the plastic through charge interactions. A solution of nonreacting protein, such as bovine serum albumin or casein , is added to each well in order to cover any plastic surface in the well ...

8. The 10 Best Podcasts Of 2024 - AOL

   www.aol.com/10-best-podcasts-2024-104523165.html

   The back episodes — featuring Johnson’s former “New Girl” co-stars Max Greenfield and Hannah Simone — are still well worth a listen for fans of comedy and questionable advice. — Liz Skalka

9. Immunostaining - Wikipedia

   en.wikipedia.org/wiki/Immunostaining

   The enzyme-linked immunosorbent assay or ELISA is a diagnostic method for quantitatively or semi-quantitatively determining protein concentrations from blood plasma, serum or cell/tissue extracts in a multi-well plate format (usually 96-wells per plate). Broadly, proteins in solution are absorbed to ELISA plates.