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The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. [ 1 ] The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber.
Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment. It is an important subset of cytometry , with applications in research and clinical practice.
Through the work of Karl von Vierordt, Louis-Charles Malassez, Karl Bürker and others blood cell concentration could by the late 19th century be accurately measured using a blood cell counting chamber, the hemocytometer, and an optical microscope. [3] [4] Until the 1950s the hemocytometer was the standard method to count blood cells. [5]
Somatic cell nuclear transfer: Used for creating a viable embryo from a body cell and an egg cell: Developmental biology: Southern blot: Used to detect specific DNA sequence in DNA samples: Molecular biology: Test cross: Used to determine whether an individual is homozygous or heterozygous dominant: Genetics: Voltage clamp
an old but rapid and simple method of hemoglobin estimation in the laboratories. Presently used in some places where sophisticated optical instruments are not available Haemocytometer: a microscope associated apparatus used for manual counting of cells in body fluids like blood, etc. including for sperm count: Wintrobe's tube
In addition to clinical counting of blood cells (cell diameters usually 6–10 micrometers), the Coulter counter has established itself as the most reliable laboratory method for counting a wide variety of cells, ranging from bacteria (<1 micrometer in size), fat cells (about 400 micrometers), stem cell embryoid bodies (about 900 micrometers ...
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A dilution of the cells to be counted is prepared and mixed with Trypan blue, this is normally the stain of choice because it is taken up by dead cells and actively excluded from live cells. Once the cells have been stained, they are counted using a hemocytometer, then a calculation is carried out to the original concentration of live cells. [1]