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  2. Cycloheximide chase - Wikipedia

    en.wikipedia.org/wiki/Cycloheximide_chase

    Cycloheximide chases are also valuable for assessing how different mutations affect the stability of a protein. Experiments have been conducted in yeast and mammalian cells to determine the critical residues required for protein stability and how disease-associated mutations may be affecting protein half-lives within the cell.

  3. N-end rule - Wikipedia

    en.wikipedia.org/wiki/N-end_rule

    The N-end rule is a rule that governs the rate of protein degradation through recognition of the N-terminal residue of proteins. The rule states that the N-terminal amino acid of a protein determines its half-life (time after which half of the total amount of a given polypeptide is degraded).

  4. Proteostasis - Wikipedia

    en.wikipedia.org/wiki/Proteostasis

    Proteostasis is the dynamic regulation of a balanced, functional proteome.The proteostasis network includes competing and integrated biological pathways within cells that control the biogenesis, folding, trafficking, and degradation of proteins present within and outside the cell.

  5. Thermal shift assay - Wikipedia

    en.wikipedia.org/wiki/Thermal_Shift_Assay

    Thermostable proteins are often more useful than their non-thermostable counterparts, e.g., DNA polymerase in the polymerase chain reaction, [7] so protein engineering often includes adding mutations to increase thermal stability. Protein crystallization is more successful for proteins with a higher melting point [8] and adding buffer ...

  6. Regulation of gene expression - Wikipedia

    en.wikipedia.org/wiki/Regulation_of_gene_expression

    Regulation of gene expression by a hormone receptor Diagram showing at which stages in the DNA-mRNA-protein pathway expression can be controlled. Regulation of gene expression, or gene regulation, [1] includes a wide range of mechanisms that are used by cells to increase or decrease the production of specific gene products (protein or RNA).

  7. Macromolecular crowding - Wikipedia

    en.wikipedia.org/wiki/Macromolecular_crowding

    The study of biochemical processes under realistically crowded conditions is very important, since these conditions are a ubiquitous property of all cells and crowding may be essential for the efficient operation of metabolism. Indeed, in vitro studies have shown that crowding greatly influences binding stability of proteins to DNA. [5]

  8. Do You Need to Increase Protein Intake As You Age? - AOL

    www.aol.com/increase-protein-intake-age...

    While researchers are still looking into why exactly we lose muscle as we age and the exact mechanisms behind decreased protein synthesis, Antonucci explains, research does show that increasing ...

  9. Protein adsorption - Wikipedia

    en.wikipedia.org/wiki/Protein_adsorption

    The increase in stability is a direct result of the observed increase in extracellular matrix and collagen attachment, which results in increased osteoblast attachment and mineralization when compared to non-roughened surfaces. [26] Adsorption is not always desirable, however.