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Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens.
Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar , resulting in the formation of a visible precipitate when they interact.
Örjan Thomas Ouchterlony (January 14, 1914, Stockholm – September 25, 2004) was a Swedish bacteriologist and immunologist who is credited with the creation of the Ouchterlony double immuno diffusion test in the 1940s. [1] [2] He was trained at Karolinska Institute, where his received his medical doctorate.
A schematic of a complement fixation test. The complement fixation test is an immunological medical test that can be used to detect the presence of either specific antibody or specific antigen in a patient's serum, based on whether complement fixation occurs.
Ouchterlony double immunodiffusion, an immunological technique named after Örjan Ouchterlony Topics referred to by the same term This disambiguation page lists articles associated with the title Ochterlony .
Immunoelectrophoresis is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. [1] Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies. The agarose was ...
Introductory video demonstrating radial immunodiffusion technique (3:43 minutes). "Radial Immunodiffusion (Mancini Technique)" (video). Frank Lectures. 2017-08-08. Archived from the original on 2021-12-21 – via YouTube. Introductory lecture/slideshow illustrating radial immunodiffusion theory and technique. (6:56 minutes)
The principles of specificity and cross-reactivity of the antigen-antibody interaction are useful in clinical laboratory for diagnostic purposes. One basic application is determination of ABO blood group. It is also used as a molecular technique for infection with different pathogens, such as HIV, microbes, and helminth parasites.