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Ion-exchange resin beads. An ion-exchange resin or ion-exchange polymer is a resin or polymer that acts as a medium for ion exchange, that is also known as an ionex. [1] It is an insoluble matrix (or support structure) normally in the form of small (0.25–1.43 mm radius) microbeads, usually white or yellowish, fabricated from an organic polymer substrate.
Examples of functional groups of Weak ion exchange resins include diethylaminoethyl (DEAE, -C 2 H 4 N(C 2 H 5) 2), which is an anion exchanger, and carboxymethyl (CM, -CH 2-COOH), [35] which is a cation exchanger. These two types of exchangers can maintain the charge density of their columns over a pH range of 5–9.
The resin is a weak exchanger because it is only partially ionized over most pH values, and an efficient separation with DEAE-C chromatography requires a specific, narrow pH range. [ 8 ] Cellulose, dextran, agarose, and other insoluble complexes are unaffected because they compose inert matrices, hence why they are so often derivatized with ...
There are also amphoteric exchangers that are able to exchange both cations and anions simultaneously. However, the simultaneous exchange of cations and anions is often performed in mixed beds, which contain a mixture of anion- and cation-exchange resins, or passing the solution through several different ion-exchange materials. Ion exchanger.
The second application example for the MCSGP prototype is the separation of three MAb variants using a preparative weak cation-exchange resin. Although the intermediately eluting MAb variant can only be obtained with 80% purity at recoveries close to zero in a batch chromatographic process, the MCSGP process can provide 90% purity at 93% yield.
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). [2] In solution, the resin is coated with positively charged counter-ions . Anion exchange resins will bind to negatively charged molecules ...
Chelating resins are a class of ion-exchange resins. They are almost always used to bind cations, and utilize chelating agents covalently attached to a polymer matrix. Chelating resins have the same bead form and polymer matrix as usual ion exchangers. Their main use is for pre-concentration of metal ions in a dilute solution.
Ion-exchange resins are polymers with a net negative or positive charge. Cation-exchange resins consist of an anionic polymer with countercations, typically Na + (sodium). The resin has a higher affinity for highly charged countercations, for example by Ca 2+ (calcium) in the case of water softening.
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