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The excess-velocity blast-yield measurement (precise measurement of the velocity of sound at the site of the explosion and then comparing it with the velocity of the blast wave) [128] provided among the most accurate measurements of the blast pressure. Another method was to use the aluminum diaphragm box gauges designed to record the peak ...
The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
The free NCBI tool Primer-BLAST integrates primer design and BLAST search into one application, [6] as do commercial software products such as ePrime and Beacon Designer. Computer simulations of theoretical PCR results ( Electronic PCR ) may be performed to assist in primer design by giving melting and annealing temperatures, etc. [ 7 ]
A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of two primer molecules that have attached to each other because of strings of complementary bases in the primers.
BLAST is more time-efficient than FASTA by searching only for the more significant patterns in the sequences, yet with comparative sensitivity. This could be further realized by understanding the algorithm of BLAST introduced below. Examples of other questions that researchers use BLAST to answer are:
The top of the College Football Playoff rankings should stay the same. But there will be change in the top 10. Our prediction for how it shakes out.
Detonating cord (also called detonation cord, detacord, detcord, blasting rope, or primer cord) is a thin, flexible plastic tube usually filled with pentaerythritol tetranitrate (PETN, pentrite). With the PETN exploding at a rate of approximately 6,400 m/s (21,000 ft/s), any common length of detonation cord appears to explode instantaneously.
1. Name a big winner from the first week of the season. Vincent Goodwill: The Oklahoma City Thunder (beating teams by nearly 20 points a night). They are looking to break the trend of a new team ...
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