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  2. Nuclear magnetic resonance spectroscopy of proteins

    en.wikipedia.org/wiki/Nuclear_magnetic_resonance...

    The NMR sample is prepared in a thin-walled glass tube. Protein nuclear magnetic resonance is performed on aqueous samples of highly purified protein. Usually, the sample consists of between 300 and 600 microlitres with a protein concentration in the range 0.1 – 3 millimolar.

  3. Varian, Inc. - Wikipedia

    en.wikipedia.org/wiki/Varian,_Inc.

    In 2008, Varian bought Oxford Diffraction, a British company specializing in X-ray diffraction equipment. On 27 July 2009, Agilent Technologies announced it would buy Varian Inc, for $1.5 Billion. On 14 October 2014, Agilent made the strategic decision to close its NMR business. Agilent entered the NMR business in 2010, with the acquisition of ...

  4. Nuclear magnetic resonance - Wikipedia

    en.wikipedia.org/wiki/Nuclear_magnetic_resonance

    Bruker 700 MHz nuclear magnetic resonance (NMR) spectrometer. Nuclear Magnetic Resonance (NMR) basic principles. Nuclear magnetic resonance (NMR) is a physical phenomenon in which nuclei in a strong constant magnetic field are disturbed by a weak oscillating magnetic field (in the near field [1]) and respond by producing an electromagnetic signal with a frequency characteristic of the magnetic ...

  5. Benchtop nuclear magnetic resonance spectrometer - Wikipedia

    en.wikipedia.org/wiki/Benchtop_nuclear_magnetic...

    X-Pulse also has options for flow NMR and a variable temperature probe allowing the measurement of samples in NMR tubes at temperatures from 20 °C to 60 °C. The magnet and spectrometer are in two separate boxes with the magnet weighing 149 kg [26] and the electronics weighing 22 kg. X-Pulse requires a standard mains electrical supply and uses ...

  6. Triple-resonance nuclear magnetic resonance spectroscopy

    en.wikipedia.org/wiki/Triple-resonance_nuclear...

    The second method is by NMR, which began in the 1980s when Kurt Wüthrich outlined the framework for NMR structure determination of proteins and solved the structure of small globular proteins. [5] The early method of structural determination of protein by NMR relied on proton-based homonuclear NMR spectroscopy in which the size of the protein ...

  7. Collaborative Computing Project for NMR - Wikipedia

    en.wikipedia.org/wiki/Collaborative_Computing...

    The Collaborative Computing Project for NMR (CCPN) is a project that aims to bring together computational aspects of the scientific community involved in NMR spectroscopy, especially those who work in the field of protein NMR. The general aims are to link new and existing NMR software via a common data standard and provide a forum within the ...

  8. Methods to investigate protein–protein interactions - Wikipedia

    en.wikipedia.org/wiki/Methods_to_investigate...

    Whereas the concept of water activity is widely known and utilized in the applied biosciences, its complement—the protein activity which quantitates proteinprotein interactions—is much less familiar to bioscientists as it is more difficult to determine in dilute solutions of proteins; protein activity is also much harder to determine for ...

  9. Paramagnetic nuclear magnetic resonance spectroscopy

    en.wikipedia.org/wiki/Paramagnetic_nuclear...

    Paramagnetism diminishes the resolution of an NMR spectrum to the extent that coupling is rarely resolved. Nonetheless spectra of paramagnetic compounds provide insight into the bonding and structure of the sample. For example, the broadening of signals is compensated in part by the wide chemical shift range (often 200 ppm in 1 H NMR).