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Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
Gene length: Longer genes will have more fragments/reads/counts than shorter genes if transcript expression is the same. This is adjusted by dividing the FPM by the length of a feature (which can be a gene, transcript, or exon), resulting in the metric fragments per kilobase of feature per million mapped reads (FPKM). [90]
In bioinformatics, sequence assembly refers to aligning and merging fragments from a longer DNA sequence in order to reconstruct the original sequence. [1] This is needed as DNA sequencing technology might not be able to 'read' whole genomes in one go, but rather reads small pieces of between 20 and 30,000 bases, depending on the technology used. [1]
The range of a particular marker refers to variety of bands it can map. A "high" range refers to relatively large fragments (measured in kb) while a "low" range refers to markers that distinguish between small fragments (measured in bp). Some markers can even be described as "ultra-low range", [16] but even more precise is the microRNA marker ...
The level of unique gene expression is represented by the count of transcripts present per million molecules, similar to SAGE output. A significant advantage is the larger library size compared with SAGE. An MPSS library typically holds 1 million signature tags, which is roughly 20 times the size of a SAGE library.
The resultant fragments are separated by size (such as electrophoresis). [1] A partial human STR profile obtained using the Applied Biosystems Identifiler kit Short tandem repeat ( STR ) analysis is a common molecular biology method used to compare allele repeats at specific loci in DNA between two or more samples.
In simple terms, per Farley, ChatGPT is a chatbot that can take human language, put it into an artificial intelligence system, and transform a mountain of information into a clear, organized, and ...
The word count is the number of words in a document or passage of text. Word counting may be needed when a text is required to stay within certain numbers of words. This may particularly be the case in academia, legal proceedings, journalism and advertising. Word count is commonly used by translators to determine the price of a translation job.