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Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Structure of a plant cell. Plant cells are the cells present in green plants, photosynthetic eukaryotes of the kingdom Plantae.Their distinctive features include primary cell walls containing cellulose, hemicelluloses and pectin, the presence of plastids with the capability to perform photosynthesis and store starch, a large vacuole that regulates turgor pressure, the absence of flagella or ...
These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. [8] Thus, in its broader sense, "tissue culture" is often used interchangeably with "cell culture".
Traditionally, plant stem cells were thought to only exist in SAM and RAM and studies were conducted based on this assumption. However, recent studies have indicated that (pro)cambium also serves as a niche for plant stem cells: "Procambium cells fulfill the criteria for being stem cells since they have the capacity for long-term self renewal and being able to differentiate into one or more ...
Unlike animal cells, almost every plant cell is surrounded by a polysaccharide cell wall. Neighbouring plant cells are therefore separated by a pair of cell walls and the intervening middle lamella, forming an extracellular domain known as the apoplast. Although cell walls are permeable to small soluble proteins and other solutes, plasmodesmata ...
Some eukaryotic cells (plant cells and fungal cells) also have a cell wall. Inside the cell is the cytoplasmic region that contains the genome (DNA), ribosomes and various sorts of inclusions. [2] The genetic material is freely found in the cytoplasm. Prokaryotes can carry extrachromosomal DNA elements called plasmids, which are usually circular.
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Comparatively, in the laboratory, most cultured cells—which are large compared to normal plant cells—produce very long and abundant stromules that extend to the cell periphery. In 2014, evidence was found of the possible loss of plastid genome in Rafflesia lagascae , a non-photosynthetic parasitic flowering plant, and in Polytomella , a ...
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