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The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...
Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]
An mpk1 deletion strain was transformed with a library of plasmid vectors containing parts of the entire yeast genome. One of the cells that grew out harbored BCK2 on its plasmid, whose overexpression rescued the mpk1 deletion phenotype. In the same publication, Bck2 was found to rescue a pck1 deletion phenotype as well. [citation needed]
Saccharomyces Genome Database is a National Institute of Health-funded research project on the Web. It provides curation of all published results on budding yeast (aka. bakers, brewers, and wine yeast) genes and their products. Its current URL is yeastgenome.org. [165] [166]
petite (ρ–) is a mutant first discovered in the yeast Saccharomyces cerevisiae.Due to the defect in the respiratory chain, 'petite' yeast are unable to grow on media containing only non-fermentable carbon sources (such as glycerol or ethanol) and form small colonies when grown in the presence of fermentable carbon sources (such as glucose).
Print/export Download as PDF; Printable version; ... "High quality de novo sequencing and assembly of the Saccharomyces arboricolus genome".
This is the process that was initially used for the Human Genome Project, however due to stability issues, YACs were abandoned for the use of bacterial artificial chromosome. The bakers' yeast S. cerevisiae is one of the most important experimental organisms for studying eukaryotic molecular genetics. [1]
The vast majority of yeast strains studied in laboratories have been altered such that they cannot perform mating type switching (by deletion of the HO gene; see below). This allows the stable propagation of haploid yeast, as haploid cells of the a mating type will remain a cells (and α cells will remain α cells), unable to form diploid cells ...