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The formation of water by the reaction and the moisture from the respiration also act as a solvent for the reaction. Reactions in aqueous phase are generally faster than between a dry gas and a dry solid. Soda lime is commonly used in closed-circuit diving rebreathers and in the anesthesia breathing circuit in anesthesia systems. [8] [9]
Huff and Puff Apparatus respiration demonstration. The huff and puff apparatus is used in school biology labs to demonstrate that carbon dioxide is a product of respiration. A pupil breathes in and out of the middle tube. The glass tubing is arranged in such a way that one flask bubbles as the pupils breathes in, the other as the pupil breathes ...
Calcium hydroxide (traditionally called slaked lime) is an inorganic compound with the chemical formula Ca 2. It is a colorless crystal or white powder and is produced when quicklime (calcium oxide) is mixed with water. Annually, approximately 125 million tons of calcium hydroxide are produced worldwide. [8]
The indicator is used in photosynthesis and respiration experiments to find out whether carbon dioxide is being liberated. [1] It is also used to test the carbon dioxide content during gaseous exchange of organisms. When the carbon dioxide content is higher than 0.04%, the initial red colour changes to yellow as the pH becomes more acidic.
A simple whole plant respirometer designed to measure oxygen uptake or CO 2 release consists of a sealed container with the living specimen together with a substance to absorb the carbon dioxide given off during respiration, such as soda lime pellets or cotton wads soaked with potassium hydroxide. The oxygen uptake is detected by manometry. [5]
Triphenyl tetrazolium chloride (TTC), or simply tetrazolium chloride (with the formula 2,3,5-triphenyl-2H-tetrazolium chloride) is a redox indicator commonly used in biochemical experiments especially to indicate cellular respiration. It is a white crystalline powder, soluble in water, ethanol and acetone but insoluble in ether.
Respirometry depends on a "what goes in must come out" principle. [6] Consider a closed system first. Imagine that we place a mouse into an air-tight container. The air sealed in the container initially contains the same composition and proportions of gases that were present in the room: 20.95% O 2, 0.04% CO 2, water vapor (the exact amount depends on air temperature, see dew point), 78% ...
In practice, doses of doubly labeled water for metabolic work are prepared by simply mixing a dose of deuterium oxide (heavy water) (90 to 99%) with a second dose of H 2 18 O, which is water which has been separately enriched with 18 O (though usually not to a high level, since doing this would be expensive, and unnecessary for this use), but ...