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The tears are unique among body fluids in that they are exposed to the environment. Much like other body fluids, tear fluid is kept in a tight pH range using the bicarbonate buffer system. [15] The pH of tears shift throughout a waking day, rising "about 0.013 pH units/hour" until a prolonged closed-eye period causes the pH to fall again. [15]
Buffers typically consist of a pair of compounds in solution, one of which is a weak acid and the other a weak base. [13] The most abundant buffer in the ECF consists of a solution of carbonic acid (H 2 CO 3), and the bicarbonate (HCO − 3) salt of, usually, sodium (Na +). [5] Thus, when there is an excess of OH −
The majority of biological samples that are used in research are kept in a buffer solution, often phosphate buffered saline (PBS) at pH 7.4. In industry, buffering agents are used in fermentation processes and in setting the correct conditions for dyes used in colouring fabrics.
Since biological cells contain fluid that can act as a buffer, pH i can be maintained fairly well within a certain range. [11] Cells adjust their pH i accordingly upon an increase in acidity or basicity, usually with the help of CO 2 or HCO 3 – sensors present in the membrane of the cell. [3]
Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
Good's buffers (also Good buffers) are twenty buffering agents for biochemical and biological research selected and described by Norman Good and colleagues during 1966–1980. [ 1 ] [ 2 ] [ 3 ] Most of the buffers were new zwitterionic compounds prepared and tested by Good and coworkers for the first time, though some ( MES , ADA , BES , Bicine ...
Total body water in healthy adults is about 50–60% (range 45 to 75%) of total body weight; [1] women and the obese typically have a lower percentage than lean men. [2] Extracellular fluid makes up about one-third of body fluid, the remaining two-thirds is intracellular fluid within cells. [3]
In lean healthy adult men, the total body water is about 60% (60–67%) of the total body weight; it is usually slightly lower in women (52–55%). [2] [3] The exact percentage of fluid relative to body weight is inversely proportional to the percentage of body fat. A lean 70 kg (150 lb) man, for example, has about 42 (42–47) liters of water ...
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