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In this technique, sodium bisulfite deaminates cytosine into uracil, but does not affect 5-methylcytosine, a methylated form of cytosine with a methyl group attached to carbon 5. When the bisulfite-treated DNA is amplified via polymerase chain reaction , the uracil is amplified as thymine and the methylated cytosines are amplified as cytosine.
The pH range is commonly given as zero to 14, but a pH value can be less than 0 for very concentrated strong acids or greater than 14 for very concentrated strong bases. [2] The pH scale is traceable to a set of standard solutions whose pH is established by international agreement. [3]
A calcium bisulfite liquor solution is used in the process of converting dihydroquercetin in tree bark pulp and then converting dihydroquercetin to a usable form: quercetin. Calcium bisulfite is not the optimum bisulfite compound for this reaction since the calcium ions can be removed from the calcium bisulfite solution during the reaction ...
It appears in form of white or yellowish-white crystals with an odor of sulfur dioxide. Regardless of its ill-defined nature, sodium bisulfite is used in many different industries such as a food additive with E number E222 in the food industry, a reducing agent in the cosmetic industry, and a decomposer of residual hypochlorite used in the ...
The indicator is used in photosynthesis and respiration experiments to find out whether carbon dioxide is being liberated. [1] It is also used to test the carbon dioxide content during gaseous exchange of organisms. When the carbon dioxide content is higher than 0.04%, the initial red colour changes to yellow as the pH becomes more acidic.
In and of themselves, pH indicators are usually weak acids or weak bases. The general reaction scheme of acidic pH indicators in aqueous solutions can be formulated as: HInd (aq) + H 2 O (l) ⇌ H 3 O + (aq) + Ind − (aq) where, "HInd" is the acidic form and "Ind −" is the conjugate base of the indicator. Vice versa for basic pH indicators ...
Phenolphthalein adopts different forms in aqueous solution depending on the pH of the solution. [4] [2] [5] [6] Inconsistency exists in the literature about hydrated forms of the compounds and the color of sulfuric acid. Wittke reported in 1983 that it exists in protonated form (H 3 In +) under strongly acidic conditions, providing an orange ...
Intracellular pH (pH i) is the measure of the acidity or basicity (i.e., pH) of intracellular fluid. The pH i plays a critical role in membrane transport and other intracellular processes. In an environment with the improper pH i , biological cells may have compromised function.