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A colorimeter is a device used to test the concentration of a solution by measuring its absorbance of a specific wavelength of light. To use this device, different solutions must be made, and a control (usually a mixture of distilled water and another solution) is first filled into a cuvette and placed inside a colorimeter to calibrate the machine.
For calibrating the monitor a colorimeter is attached flat to the display's surface, shielded from all ambient light. The calibration software sends a series of color signals to the display and compares the values that were actually sent against the readings from the calibration device. This establishes the current offsets in color display.
A colorimeter is a device used in colorimetry that measures the absorbance of particular wavelengths of light by a specific solution. [1] [2] It is commonly used to determine the concentration of a known solute in a given solution by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance.
The equipment required is a colorimeter, some cuvettes and a suitable color reagent. The process may be automated, e.g. by the use of an AutoAnalyzer or by flow injection analysis . Recently, colorimetric analyses developed for colorimeters have been adapted for use with plate readers to speed up analysis and reduce the waste stream.
A tristimulus colorimeter measures the tristimulus values of a color. [4] A spectroradiometer measures the absolute spectral radiance (intensity) or irradiance of a light source. [5] A spectrophotometer measures the spectral reflectance, transmittance, or relative irradiance of a color sample. [5] [6]
In this calibration, the transmittance is set at 100% using the calibration knob of the instrument (the amplifier control knob in the figure at right). The instrument can also optionally be calibrated with a stock solution of a sample at a concentration known to have an absorbance of 2 or else vendor supplied standards, using the light ...
A colorimeter or a digital camera with a color filter array can, under certain conditions, be used as an alternative to a spectrophotometer. [7] [8] The illuminant and observer conditions should be specified when citing a measurement (e.g. D65/10°). [9] The quality of a colorimeter may be assessed using the means in CIE publication 179:2007. [10]
A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
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