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Due to the extremely low amounts of liquids 0.1 - 10 nl and reagents, the methods to work with nanoliterplates are completely different compared to the methods used for microliter plates. Nanoliter plates reduce the amount of reagents, require lower sample volumes and increase the numbers of tests that can be performed in the lab .
The integrated nanoliter system consists of microfabricated fluidic channels, heaters, temperature sensors, and fluorescence detectors. The microfabricated fluidic channels (basically very small pipes) act as the main transportation structures for any fluids as well as where reactions occur within the system.
Droplet Digital PCR (ddPCR) is a method of dPCR in which a 20 microliter sample reaction including assay primers and either Taqman probes or an intercalating dye, is divided into ~20,000 nanoliter-sized oil droplets through a water-oil emulsion technique, thermocycled to endpoint in a 96-well PCR plate, and fluorescence amplitude read for all ...
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The medical examiner who ruled the 2011 death of a Philadelphia teacher found with 20 stab wounds a homicide — then later a suicide — now says he believes the case should be ruled as ...
Today's NYT Connections puzzle for Thursday, February 6, 2025The New York Times
Silicone rubber and glass microfluidic devices. Top: a photograph of the devices. Bottom: Phase contrast micrographs of a serpentine channel ~15 μm wide. The behaviour of fluids at the microscale can differ from "macrofluidic" behaviour in that factors such as surface tension, energy dissipation, and fluidic resistance start to dominate the system.