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There is much more phosphotungstic acid in the solution than hematein. The phosphotungstic acid binds all of the available hematein to form a blue lake pigment. This lake stains the muscle cross striations, fibrin, nuclei, and other tissue elements blue. The rest of the phosphotungstic acid stains the red-brown components, such as collagen. [2]
Dilated peri-tubular capillaries filled with sickled RBCs, original Gomori's trichrome stain, × 400. [1] The "ragged red fibers" in MELAS syndrome are visible under modified Gomori stain. Gömöri trichrome stain is a histological stain used on muscle tissue. [2] [3] It can be used to test for certain forms of mitochondrial myopathy.
Mouse skin stained with Masson's trichrome stain. Masson's trichrome is a three-colour staining procedure used in histology . The recipes emerged from Claude L. Pierre Masson 's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue .
Trichrome stains in which dyes and a polyacid are applied sequentially are called multi-step trichromes. In "one-step" methods, all the dyes—with or without a polyacid—are combined in a single solution. One of the oldest single-step approaches to trichrome staining is van Gieson's method, which stains muscle and cytoplasm yellow, and ...
Hematoxylin staining shown as "basophilic" at top, seen with dual staining with hematoxylin and eosin (H&E). Haematoxylin stain is commonly followed (or counterstained) with another histologic stain, eosin. [10] [11] [1] When paired, this staining procedure is known as H&E staining, and is one of the most commonly used combinations in histology.
Positive histologic stains that aid in the diagnosis of conditions of or affecting the human integumentary system Stain Cell, material, and/or structure(s) stained Condition(s) in which stain is positive Actin-specific enolase: Infantile digital fibromatosis: AE1/AE3: Squamous cell carcinoma: Alcian blue: Lipoid proteinosis Papular mucinosis ...
Van Gieson's stain in an angioleiomyoma, making smooth muscle fibers yellow and collagen fibers red. Hematoxylin and Van Gieson's stain gives collagen a pink color, such as in fibrosis (arrows, here in cirrhosis). Van Gieson's stain is a mixture of picric acid and acid fuchsin.
A commonly performed histochemical technique that targets a specific chemical is the Perls' Prussian blue reaction, used to demonstrate iron deposits [12] in diseases like hemochromatosis. The Nissl method for Nissl substance and Golgi's method (and related silver stains ) are useful in identifying neurons are other examples of more specific ...