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  2. Primer (paint) - Wikipedia

    en.wikipedia.org/wiki/Primer_(paint)

    A layer of primer will prevent the underlying wood from prematurely absorbing the solvents in the finishing paint. Primer reduces the number of paint coats needed for good coverage and even color. A thin layer of paint may still be permeable to water. Water can permeate into the wood and cause warping, mildew, or dry rot.

  3. Gesso - Wikipedia

    en.wikipedia.org/wiki/Gesso

    A restored gesso panel representing St. Martin of Tours, from St. Michael and All Angels Church, Lyndhurst, Hampshire. Gesso (Italian pronunciation:; 'chalk', from the Latin: gypsum, from Greek: γύψος), also known as "glue gesso" or "Italian gesso", [1] is a white paint mixture used to coat rigid surfaces such as wooden painting panels or masonite as a permanent absorbent primer substrate ...

  4. Stain-blocking primer - Wikipedia

    en.wikipedia.org/wiki/Stain-blocking_primer

    A good primer has to be compatible with a wide variety of substrates that may be encountered in an interior situation such as: drywall, cement, concrete, plaster and spackling, wood, paneling, old paint, metals, fiberboard, etc. Very frequently, particularly in renovation work, the surfaces encountered will be covered with a variety of stains ...

  5. Primer dimer - Wikipedia

    en.wikipedia.org/wiki/Primer_dimer

    A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of two primer molecules that have attached ( hybridized ) to each other because of strings of complementary bases in the primers.

  6. TOPO cloning - Wikipedia

    en.wikipedia.org/wiki/TOPO_Cloning

    The insert is created by PCR using Taq DNA polymerase, a polymerase that lacks 3' to 5' proofreading activity and with a high probability adds a single, 3'-adenine overhang to each end of the PCR product. It is best if the PCR primers have guanines at the 5' end as this maximizes probability of Taq DNA polymerase adding the terminal adenosine ...

  7. Nested polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Nested_polymerase_chain...

    Polymerase chain reaction itself is the process used to amplify DNA samples, via a temperature-mediated DNA polymerase.The products can be used for sequencing or analysis, and this process is a key part of many genetics research laboratories, along with uses in DNA fingerprinting for forensics and other human genetic cases.

  8. Variants of PCR - Wikipedia

    en.wikipedia.org/wiki/Variants_of_PCR

    Adjustments to the synthetic oligonucleotides used as primers in PCR are a rich source of modification: Normally PCR primers are chosen from an invariant part of the genome, and might be used to amplify a polymorphic area between them. In allele-specific PCR the opposite is done. At least one of the primers is chosen from a polymorphic area ...

  9. Touchdown polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Touchdown_polymerase_chain...

    The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing. The melting point of the primer sets the upper limit on annealing temperature. At temperatures just above this point, only very specific base pairing between the primer and the template will occur.

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