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The DyLight Fluor family of fluorescent dyes are produced by Dyomics in collaboration with Thermo Fisher Scientific. [4] DyLight dyes are typically used in biotechnology and research applications as biomolecule , cell and tissue labels for fluorescence microscopy , cell biology or molecular biology .
S. cerevisiae septins revealed with fluorescent microscopy utilizing fluorescent labeling. In molecular biology and biotechnology, a fluorescent tag, also known as a fluorescent label or fluorescent probe, is a molecule that is attached chemically to aid in the detection of a biomolecule such as a protein, antibody, or amino acid.
Fluorescent sea dye. Fluorescent dyes find a wide use in industry, going under the name of "neon colors", such as: Multi-ton scale usages in textile dyeing and optical brighteners in laundry detergents; Advanced cosmetic formulations; Safety equipment and clothing; Organic light-emitting diodes (OLEDs) Fine arts and design (posters and paintings)
Laser-induced fluorescence (LIF) or laser-stimulated fluorescence (LSF) [1] is a spectroscopic method in which an atom or molecule is excited to a higher energy level by the absorption of laser light followed by spontaneous emission of light. [2] [3] It was first reported by Zare and coworkers in 1968. [4] [5]
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
Fluorescent minerals emit visible light when exposed to ultraviolet. Fluorescent marine organisms Fluorescent clothes used in black light theater production, Prague. Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation.
The expanded beam of a laser is focused in only one direction by a cylindrical lens, or by a combination of a cylindrical lens and a microscope objective as the latter is available in better optical quality and with higher numerical aperture than the first. This way a thin sheet of light or lightsheet is created in the focal region that can be ...
A ring dye laser. P-pump laser beam; G-gain dye jet; A-saturable absorber dye jet; M0, M1, M2-planar mirrors; OC–output coupler; CM1 to CM4-curved mirrors. A dye laser uses a gain medium consisting of an organic dye, which is a carbon-based, soluble stain that is often fluorescent, such as the dye in a highlighter pen.
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