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Aqueous ferric chloride was added to a urine sample, and the formation of the iron complex turned the solution purple. [2] This test was not specific to acetylsalicylic acid but would occur in the presence of any phenol or enol. The downfall of this test occurs in the presence of hyperbilirubinemia or elevated bilirubin.
The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols , hydroxamic acids , oximes, and sulfinic acids give positive results as well. [ 1 ]
The Trinder solution/reagent is a pre-mixed solution of 10% ferric chloride. [1] It can be prepared by combining 40 g of mercuric chloride and 40 g of ferric nitrate in 850 ml of type II deionized water, and then adding 10 ml of concentrated hydrochloric acid to the solution and diluting to a volume of 1 litre with more type II deionized water ...
Another method of visualizing the Keller-Kiliani reaction is to treat the test solution with ferric chloride-containing glacial acetic acid, followed by the addition of concentrated sulfuric acid, which sinks to the bottom (like in the brown ring test for nitrates). A brown ring in the interface indicates the presence of cardenolides.
Ferric chloride (FeCl 3) test is also a colorimetric assay. Lamaison and Carnet have designed a test for the determination of the total flavonoid content of a sample (AlCI 3 method). After proper mixing of the sample and the reagent, the mixture is incubated for 10 minutes at ambient temperature and the absorbance of the solution is read at 440 nm.
The reaction is used in a chemical test for the detection of aldehydes in combination with ferric chloride. In this test a few drops of aldehyde containing specimen is dissolved in ethanol, the sulfonamide is added together with some sodium hydroxide solution and then the solution is acidified to Congo red. An added drop of ferric chloride will ...
The excess phenylpyruvic acid in the urine of an individual with PKU would produce a bright green colour when reacting with the ferric chloride. Infants do not excrete high enough concentrations of this compound to give a positive test result, thus delaying their diagnosis, and allowing irreversible damage to take place. [1]
Ferric chloride test (detects abnormal metabolites in urine) Ninhydrin paper chromatography (detects abnormal amino acid patterns) Guthrie test (detects excessive amounts of specific amino acids in blood) The dried blood spot can be used for multianalyte testing using Tandem Mass Spectrometry (MS/MS). This given an indication for a disorder.