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Simmons’ citrate agar was developed by James S. Simmons in 1926 by adding 1.5% agar and bromothymol blue as a pH indicator to Koser’s citrate agar to observe changes in pH as a result of oxidative reactions from citrate metabolism. [5]
The citrate test detects the ability of an ... Bacterial colonies are picked up from a straight wire and inoculated into slope of Simmons citrate agar and incubated ...
The agar contains citrate and ammonium ions (nitrogen source) and bromothymol blue (BTB) as a pH indicator. [2] Bromothymol blue was added in order to reduce false positives. The citrate agar is green before inoculation, and turns blue, because of BTB as a positive test indicator, meaning citrate is utilized.
DCA agar (deoxycholate citrate agar) is a solid bacteriological growth medium used for isolation of enteric pathogens. [1] Uses
Apocholate citrate agar (ACA) is a selective environment used to isolate Shigella and Salmonella bacteria. [1] The name derives from apocholate and citrate in agar.
Stab Cultures after inoculation by inoculation needle with E. coli from left to right: TSI, Soft nutrient, Simmons Citrate agar. Inoculation of the microorganisms will be done directly after the transfer from culture to inoculation needle. The inoculum is commonly inoculated to broth cultures, slant cultures, plate cultures, and stab cultures.
Thiosulfate–citrate–bile salts–sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species. [1] TCBS agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other Vibrio species.
Contamination on an agar plate. An agar plate is a Petri dish that contains a growth medium solidified with agar, used to culture microorganisms. Sometimes selective compounds are added to influence growth, such as antibiotics. [1] 96 pinner used to perform spot assays with yeast, fungal or bacterial cells