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Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...
As with binoculars and telescopes, monoculars are primarily defined by two parameters: magnification and objective lens diameter, for example, 8×30 where 8 is the magnification and 30 is the objective lens diameter in mm (this is the lens furthest from the eye). An 8× magnification makes the distant object appear to be 8 times larger at the eye.
The maximum angular magnification (compared to the naked eye) of a magnifying glass depends on how the glass and the object are held, relative to the eye. If the lens is held at a distance from the object such that its front focal point is on the object being viewed, the relaxed eye (focused to infinity) can view the image with angular ...
The actual power or magnification of a compound optical microscope is the product of the powers of the eyepiece and the objective lens. For example a 10x eyepiece magnification and a 100x objective lens magnification gives a total magnification of 1,000×.
Field of view is the area of the inspection captured on the camera’s imager. The size of the field of view and the size of the camera’s imager directly affect the image resolution (one determining factor in accuracy). Working distance is the distance between the back of the lens and the target object.
So a standard 50 mm lens for 35 mm photography acts like a 50 mm standard "film" lens even on a professional digital SLR, but would act closer to a 75 mm (1.5×50 mm Nikon) or 80 mm lens (1.6×50mm Canon) on many mid-market DSLRs, and the 40-degree angle of view of a standard 50 mm lens on a film camera is equivalent to a 28–35 mm lens on ...
For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives good optical sectioning. High magnification objective lenses typically have higher numerical apertures (and so better optical sectioning) than low magnification objectives.
The ratio of the focal length of the objective and the eyepiece, when mounted in a standard tube length, gives an approximate magnification of the system. Due to their design, compound microscopes have improved resolving power and contrast in comparison to simple microscopes, [ 11 ] and can be used to view the structure, shape and motility of a ...