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HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent.It is one of the twenty Good's buffers.HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in ...
Good's buffers (also Good buffers) are twenty buffering agents for biochemical and biological research selected and described by Norman Good and colleagues during 1966–1980. [ 1 ] [ 2 ] [ 3 ] Most of the buffers were new zwitterionic compounds prepared and tested by Good and coworkers for the first time, though some ( MES , ADA , BES , Bicine ...
HEPBS (N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid)) is a zwitterionic organic chemical buffering agent; one of Good's buffers.HEPBS and HEPES have very similar structures and properties, HEPBS also having an acidity (pK a) in the physiological range (7.6-9.0 useful range).
The optimal day temperature range for cannabis is 24 to 30 °C (75 to 86 °F). [12] Temperatures above 31 °C (88 °F) and below 15.5 °C (60 °F) seem to decrease THC potency and slow growth. At 13 °C (55 °F) the plant undergoes a mild shock, though some strains withstand frost temporarily.
Buffer capacity falls to 33% of the maximum value at pH = pK a ± 1, to 10% at pH = pK a ± 1.5 and to 1% at pH = pK a ± 2. For this reason the most useful range is approximately pK a ± 1. When choosing a buffer for use at a specific pH, it should have a pK a value as close as possible to that pH. [2]
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES, [1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring.
In hydroponic growing, the nutrient solution is best at 5.2 to 5.8, making Cannabis well-suited to hydroponics because this pH range is hostile to most bacteria and fungi. [ citation needed ] Tissue culture multiplication has become important in producing medically important clones, [ 18 ] while seed production remains the generally preferred ...
For this reason, DEPC cannot be used with Tris or HEPES buffers. In contrast, it can be used with phosphate-buffered saline or MOPS. [3] A handy rule is that enzymes or chemicals which have active -O:, -N: or -S: cannot be treated with DEPC to become RNase-free, as DEPC reacts with these species.
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