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The protonated form of bromothymol blue has its peak absorption at 427 nm thus transmitting yellow light in acidic solutions, while the deprotonated form has its peak absorption at 602 nm thus transmitting blue light in more basic solutions. [3] In contrast, highly acidic bromothymol blue is magenta in color.
Solution: The main components of a universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue, and phenolphthalein. This mixture is important because each component loses or gains protons depending upon the acidity or alkalinity of the solution being tested. It is beneficial to use this type of universal ...
Bromothymol blue is the indicator used in the agar, it changes to yellow in case of acid production during fermentation of lactose or changes to deep blue in case of alkalinization. Lactose-positive bacteria build yellow colonies. Bacteria which decarboxylate L-cystine cause an alkaline reaction and build deep blue colonies. [1]
yellow Thymol blue (second transition) yellow 8.0 9.6 blue Methyl yellow: red 2.9 4.0 yellow Methylene blue: colorless 5.0 9.0 dark blue Bromophenol blue: yellow 3.0 4.6 blue Congo red: blue-violet 3.0 5.0 red Methyl orange: red 3.1 4.4 yellow Screened methyl orange (first transition) red 0.0 3.2 purple-grey Screened methyl orange (second ...
The increase in pH then causes color change in the bromothymol blue indicator, turning it blue. Under neutral conditions the medium remains a green color. The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change.
Sucrose fermentation produces acid, which converts the colour of bromothymol blue or thymol blue. Two dyes rather than one make the medium produce an array of yellow, green, or blue so that differentiating among various Vibrio species is possible.
The standards used include potassium dichromate (isosbestic points at 339 and 445 nm), bromothymol blue (325 and 498 nm) and congo red (541 nm). The wavelength of the isosbestic point determined does not depend on the concentration of the substance used, and so it becomes a very reliable reference.
The Sigma-Aldrich catalog lists the pKa value of 7.10 as approximate. (The quoted description from the online catalog is: Bromothymol Blue (BTB) is a pH indicator (pKa approx. 7.1) that is used as a biological slide vital stain to define cell walls or nuclei under the microscope.
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