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Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. [1] This takes place through a pilus .
In bacterial conjugation, DNA is transferred via cell-to-cell communication. Cell-to-cell communication may involve plasmids that allow for the transfer of DNA into another neighboring cell. [ 19 ] The neighboring cells absorb the F-plasmid (fertility plasmid: inherited material that is present in the chromosome).
Conjugation in bacteria using a sex pilus; then the bacterium that received the plasmid can go give it to other bacteria as well. E. coli cells going through conjugation and sharing genetic information. F-pilus is reaching towards other cell. As mentioned before, conjugation is a method of horizontal gene transfer through cell to cell contact. [43]
During conjugation, a pilus emerging from the donor bacterium ensnares the recipient bacterium, draws it in close, and eventually triggers the formation of a mating bridge, which establishes direct contact and the formation of a controlled pore that allows transfer of DNA from the donor to the recipient.
The transferred DNA (called T-DNA) is piloted to the plant cell nucleus by nuclear localization signals present in the Agrobacterium protein VirD2, which is covalently attached to the end of the T-DNA at the Right border (RB). Exactly how the T-DNA is integrated into the host plant genomic DNA is an active area of plant biology research.
In a typical conjugation, the recipient cell also becomes F + after conjugation as it receives an entire copy of the F factor plasmid; but this is not the case in conjugation mediated by Hfr cells. Due to the large size of bacterial chromosome, it is very rare for the entire chromosome to be transferred into the F − cell as time required is ...
In bacterial conjugation, where DNA is transferred between bacteria through direct cell-to-cell contact, homologous recombination helps integrate foreign DNA into the host genome via the RecBCD pathway. The RecBCD enzyme promotes recombination after DNA is converted from single-strand DNA–in which form it originally enters the bacterium–to ...
The nucleophilic lysine residue is commonly targeted site in protein bioconjugation, typically through amine-reactive N-hydroxysuccinimidyl (NHS) esters. [3] To obtain optimal number of deprotonated lysine residues, the pH of the aqueous solution must be below the pKa of the lysine ammonium group, which is around 10.5, so the typical pH of the reaction is about 8 and 9.