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An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
The first culture media was liquid media, designed by Louis Pasteur in 1860. [2] This was used in the laboratory until Robert Koch's development of solid media in 1881. [3] Koch's method of using a flat plate for his solid media was replaced by Julius Richard Petri's round box in 1887. [2]
Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton, who were microbiologists working at Harvard University.
Sabouraud agar is used to culture fungi and has a low pH that inhibits the growth of most bacteria; it also contains the antibiotic gentamicin to specifically inhibit the growth of Gram-negative bacteria. Hay infusion agar is specific for the culturing of slime moulds (which are not fungi). Potato dextrose agar is used to culture certain types ...
These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium. [3] Since its development in 1985, [3] it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media. [citation needed]
LB medium bottle and LB agar plate Plate medium agar LB. Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani, intended LB to stand for lysogeny broth, [1] but LB has also come to colloquially mean Luria broth, Lennox broth, life broth or Luria–Bertani medium. [2]
Streak the mixed culture back and forth in the first quadrant (top left) of the agar plate. Do not cut the agar, simply scrape the top. Flame the loop to rid of culture residue. Wait for it to cool for the next quadrant. Streaking again. Proceed to the second quadrant with streaking. Streaks on the medium will overlap.
CNA agar is commonly used in clinical microbiology laboratories to isolate pathogenic Gram-positive bacteria such as Staphylococcus, Enterococcus, Streptococcus, diphtheroids, and Listeria from clinical specimens. [1] A common use for CNA agar is commonly used for the detection of Streptococcus agalactiae carriage in pregnant women. [2]