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Matrices of varying stiffness are commonly engineered for experimental and therapeutic purposes (e.g. collagen matrices for wound healing [24]). Durotactic gradients are simply made by creating 2-dimensional substrates out of polymer (e.g. acrylamide [ 13 ] or polydimethylsiloxane ) in which the stiffness is controlled by cross-linking density ...
Micro-mass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. A commonly applied definition of tissue engineering, as stated by Langer [3] and Vacanti, [4] is "an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve [Biological tissue] function or a ...
As a result, this staining technique can reveal collagen, ordinary cytoplasm, and red blood cells. It is used in examining the collagen of connective tissue. For tissues that are not directly acidic or basic, it can be difficult to use only one stain to reveal the necessary structures of interest.
As granulation tissue matures, the fibroblasts produce less collagen and become more spindly in appearance. They begin to produce the much stronger type I collagen. Some of the fibroblasts mature into myofibroblasts which contain the same type of actin found in smooth muscle, which enables them to contract and reduce the size of the wound.
Collagen is also abundant in corneas, blood vessels, the gut, intervertebral discs, and the dentin in teeth. [3] In muscle tissue, it serves as a major component of the endomysium. Collagen constitutes 1% to 2% of muscle tissue and accounts for 6% of the weight to skeletal muscle. [4] The fibroblast is the most common cell creating collagen in ...
In one case, however, a particular type of collagen graft led to significant delay of wound closure. [6] Careful study of histology samples revealed that grafts that delayed wound closure induced the synthesis of new dermis de novo at the injury site, instead of forming scar, which is the normal outcome of the spontaneous wound healing response.
The C-terminal telopeptide (CTX), also known as carboxy-terminal collagen crosslinks, is the C-terminal telopeptide of fibrillar collagens such as collagen type I and type II. It is used as a biomarker in the serum to measure the rate of bone turnover .
Timing is important to wound healing. Critically, the timing of wound re-epithelialization can decide the outcome of the healing. [11] If the epithelization of tissue over a denuded area is slow, a scar will form over many weeks, or months; [12] [13] If the epithelization of a wounded area is fast, the healing will result in regeneration.
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