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Custom acquisition, analysis and processing plugins can be developed using ImageJ's built-in editor and a Java compiler. User-written plugins make it possible to solve many image processing and analysis problems, from three-dimensional live-cell imaging [ 6 ] to radiological image processing, [ 7 ] multiple imaging system data comparisons [ 8 ...
The addition of cycloheximide to cultured cells followed by protein lysis at multiple timepoints is conducted to observe protein degradation over time and can be used to determine a protein's half-life. These assays are often followed by western blotting to assess protein abundance and can be analyzed using quantitative tools such as ImageJ. [2]
"Analyzing gels and western blots with ImageJ". lukemiller.org "Quantification of Western Blots Using ImageJ". How to WESTERN-BLOT "Image Studio Lite Software". licor.com. Archived from the original on 3 March 2014 "ImageJ" "MCID Core Digital Imaging Software".
Sonication is the act of applying sound energy to agitate particles in a sample, for various purposes such as the extraction of multiple compounds from plants, microalgae and seaweeds. [1] Ultrasonic frequencies (> 20 kHz) are usually used, leading to the process also being known as ultrasonication or ultra-sonication .
Sonoporation, or cellular sonication, is the use of sound in the ultrasonic range for increasing the permeability of the cell plasma membrane. This technique is usually used in molecular biology and non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or ...
A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein, or for DNA extraction).
Homogenization of tissue in solution is often performed simultaneously with cell lysis.To prevent lysis however, the tissue (or collection of cells, e.g. from cell culture) can be kept at temperatures slightly above zero to prevent autolysis, and in an isotonic solution to prevent osmotic damage.
While Fiji was originally intended for neuroscientists (and continues to be so [8]), it accumulated enough functionality to attract scientists from a variety of fields, such as cell biology, [9] parasitology, [10] genetics, life sciences in general, materials science, etc.