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Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues ), in cytology (microscopic study of cells ), and in the medical fields of histopathology , hematology , and cytopathology that focus on the study and ...
It has been used as dental products as luting agent. Calcium aluminate cement (bioceramic) has been developed for dental products and root-end filling material. These newly developed root-end filling materials are based on bioceramic, chemically bonded ceramic, not by mineral (ceramic in nature) like MTA. Even if mineral shows higher ...
While in supravital staining the living cells take up the stain, in "vital staining" – the most accepted but apparently paradoxical meaning of this term, the living cells exclude the stain i.e. stain negatively and only the dead cells stain positively and thus viability can be assessed by counting the percentage of total cells that stain ...
The material is also more technique-forgiving compared to composite restorations used for that purpose. Dental amalgam is also radiopaque which is beneficial for differentiating the material between tooth tissues on radiographs for diagnosing secondary caries. The cost of the restoration is typically cheaper than composite restorations.
Internal staining is common following root canal treatment; however, the exact causes for this are not completely understood. [1] Failure to completely clean out the necrotic soft tissue of the pulp system may cause staining, and certain root canal materials (e.g., gutta percha and root canal sealer cements) can also. [1]
Anterior teeth are some of the most scrutinized teeth, as the size, shape and color of the anterior upper teeth plays an important role in dental aesthetics and smile aesthetics. [1] A few aesthetic anterior problems, such as dental caries, tooth fracture, [2] enamel defects [3] and diastemas, can be solved with composite restorations ...
Tooth discolouration and staining is primarily due to two sources of stain: intrinsic and extrinsic (see Figure 2). [1] In essence, tooth whitening primarily targets those intrinsic stains in which cannot be removed through mechanics such as a debridement (clean) or prophylaxis, in the dental office. [6]
Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. [2] While the first cases of IHC staining used fluorescent dyes (see immunofluorescence ), other non-fluorescent methods using enzymes such as peroxidase (see ...