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Carbohydrate NMR spectroscopy is the application of nuclear magnetic resonance (NMR) spectroscopy to structural and conformational analysis of carbohydrates.This method allows the scientists to elucidate structure of monosaccharides, oligosaccharides, polysaccharides, glycoconjugates and other carbohydrate derivatives from synthetic and natural sources.
The Harris–Benedict equation (also called the Harris-Benedict principle) is a method used to estimate an individual's basal metabolic rate (BMR).. The estimated BMR value may be multiplied by a number that corresponds to the individual's activity level; the resulting number is the approximate daily kilocalorie intake to maintain current body weight.
An example of a positive Seliwanoff’s test. Seliwanoff’s test is a chemical test which distinguishes between aldose and ketose sugars.If the sugar contains a ketone group, it is a ketose.
Method: Dissolve one or two crystals of cholesterol in dry chloroform in a dry test tube. Add several drops of acetic anhydride and then 2 drops of concentrated H 2 SO 4 and mix carefully. After the reaction is finished, the concentration of cholesterol can be measured using spectrophotometry .
The Atwater system, [1] named after Wilbur Olin Atwater, or derivatives of this system are used for the calculation of the available energy of foods.The system was developed largely from the experimental studies of Atwater and his colleagues in the later part of the 19th century and the early years of the 20th at Wesleyan University in Middletown, Connecticut.
Molisch test (using α-napthol) indicating a positive result (see purple ring). Molisch's test is a sensitive chemical test, named after Austrian botanist Hans Molisch, for the presence of carbohydrates, based on the dehydration of the carbohydrate by sulfuric acid or hydrochloric acid to produce an aldehyde, which condenses with two molecules of a phenol (usually α-naphthol, though other ...
Benedict's reagent is a deep-blue aqueous solution. Each litre contains: [4] 17.3 g copper sulfate; 173 g sodium citrate; 100 g anhydrous sodium carbonate or, equivalently, 270 g sodium carbonate decahydrate
This method allows estimating BEE and REE as well as identification of energy substrates that are predominantly metabolized by the body at a specific moment. It is based on the indirect measurement of the heat produced by oxidation of macronutrients , which is estimated by monitoring O 2 consumption and CO 2 production for a certain period of ...