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A potometer' (from Greek ποτό = drunken, and μέτρο = measure), sometimes known as transpirometer, is a device used for measuring the rate of water uptake of a leafy shoot which is almost equal to the water lost through transpiration. The causes of water uptake are photosynthesis and transpiration. [1]
Photosynthesis systems function by measuring gas exchange of leaves. Atmospheric carbon dioxide is taken up by leaves in the process of photosynthesis, where CO 2 is used to generate sugars in a molecular pathway known as the Calvin cycle. This draw-down of CO 2 induces more atmospheric CO 2 to diffuse through stomata into the air spaces of the ...
Photosynthesis systems use infrared gas analyzers (IRGAS) for measuring photosynthesis. CO 2 concentration changes in leaf chambers are measured to provide carbon assimilation values for leaves or whole plants. Research has shown that the rate of photosynthesis is directly related to the amount of carbon assimilated by the plant.
Photosynthetic capacity (A max) is a measure of the maximum rate at which leaves are able to fix carbon during photosynthesis. It is typically measured as the amount of carbon dioxide that is fixed per metre squared per second, for example as μmol m −2 sec −1.
The CO 2 compensation point (Γ) is the CO 2 concentration at which the rate of photosynthesis exactly matches the rate of respiration. There is a significant difference in Γ between C 3 plants and C 4 plants: on land, the typical value for Γ in a C 3 plant ranges from 40–100 μmol/mol, while in C 4 plants the values are lower at 3–10 μmol/mol. Plants with a weaker CCM, such as C2 ...
Chlorophyll fluorescence appears to be a measure of photosynthesis, but this is an over-simplification. Fluorescence can measure the efficiency of PSII photochemistry, which can be used to estimate the rate of linear electron transport by multiplying by the light intensity.
Photosynthesis measurement systems are not designed to directly measure the amount of light the leaf absorbs, but analysis of chlorophyll fluorescence, P700- and P515-absorbance, and gas exchange measurements reveal detailed information about, e.g., the photosystems, quantum efficiency and the CO 2 assimilation rates.
The following is a breakdown of the energetics of the photosynthesis process from Photosynthesis by Hall and Rao: [6]. Starting with the solar spectrum falling on a leaf, 47% lost due to photons outside the 400–700 nm active range (chlorophyll uses photons between 400 and 700 nm, extracting the energy of one 700 nm photon from each one)
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