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  2. Transmission electron cryomicroscopy - Wikipedia

    en.wikipedia.org/wiki/Transmission_electron_cryo...

    CryoTEM image of GroEL suspended in amorphous ice at 50 000 × magnification Structure of Alcohol oxidase from Pichia pastoris by CryoTEM. Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid ...

  3. Cryogenic electron microscopy - Wikipedia

    en.wikipedia.org/wiki/Cryogenic_electron_microscopy

    Cryogenic transmission electron microscopy (cryo-TEM) is a transmission electron microscopy technique that is used in structural biology and materials science. Colloquially, the term "cryogenic electron microscopy" or its shortening "cryo-EM" refers to cryogenic transmission electron microscopy by default, as the vast majority of cryo-EM is ...

  4. Cryogenic electron tomography - Wikipedia

    en.wikipedia.org/wiki/Cryogenic_electron_tomography

    In most other electron microscopy-based methods for imaging biological samples, combining the signal from many different sample copies has been the general way of surpassing this problem (e.g. crystallography, single particle analysis). In cryoET, instead of taking many images of different sample copies, many images are taken of one area.

  5. Cryomicroscopy - Wikipedia

    en.wikipedia.org/wiki/Cryomicroscopy

    Although optical microscopes have existed for centuries, cryomicroscopy is a modern methodology. In the 1950s, ice crystals were studied by installing an electron microscope inside of an igloo. [1] Circa 1980, the adaption of the electron microscope, the vacuum, and the cryostat led to the conception of the modern cryomicroscopy.

  6. Scanning electron cryomicroscopy - Wikipedia

    en.wikipedia.org/wiki/Scanning_electron_cryo...

    Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber. The cooling is usually achieved with liquid nitrogen. [1]

  7. Andor Technology - Wikipedia

    en.wikipedia.org/wiki/Andor_Technology

    In April 2020, Andor introduced the Marana 4.2B-6 back-illuminated scientific camera that provides up to -45 °C for 95% quantum performance and vacuum cooling. For dynamic imaging or spectroscopic applications, it offers up to 74 fps, such as wavefront sensing, lucky / speckle imaging, quantum gas dynamics, or hyperspectral imaging. In ...

  8. Scanning transmission electron microscopy - Wikipedia

    en.wikipedia.org/wiki/Scanning_transmission...

    Cryogenic electron microscopy in STEM (Cryo-STEM) allows specimens to be held in the microscope at liquid nitrogen or liquid helium temperatures. This is useful for imaging specimens that would be volatile in high vacuum at room temperature.

  9. Microcrystal electron diffraction - Wikipedia

    en.wikipedia.org/wiki/Microcrystal_electron...

    Microcrystal electron diffraction, or MicroED, [1] [2] is a CryoEM method that was developed by the Gonen laboratory in late 2013 at the Janelia Research Campus of the Howard Hughes Medical Institute. MicroED is a form of electron crystallography where thin 3D crystals are used for structure determination by electron diffraction. Prior to this ...

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