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Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
The original interface between capillary zone electrophoresis and mass spectrometry was developed in 1987 [9] by Richard D. Smith and coworkers at Pacific Northwest National Laboratory, and who also later were involved in development of interfaces with other CE variants, including capillary isotachophoresis and capillary isoelectric focusing.
Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube, which is filled with the analyte. High-performance liquid chromatography separates analytes by passing them, under high pressure, through a column filled with stationary phase .
The total oncotic pressure of an average capillary is about 28 mmHg with albumin contributing approximately 22 mmHg of this oncotic pressure, despite only representing 50% of all protein in blood plasma at 35-50 g/L. [6] [7] Because blood proteins cannot escape through capillary endothelium, oncotic pressure of capillary beds tends to draw ...
A blood gas test or blood gas analysis tests blood to measure blood gas tension values and blood pH.It also measures the level and base excess of bicarbonate.The source of the blood is reflected in the name of each test; arterial blood gases come from arteries, venous blood gases come from veins and capillary blood gases come from capillaries. [1]
Individual instruments, of a particular model type, have also been known to differ in performance (e.g. differences among various ABI 310 units, all of the same model). Capillary electrophoresis instruments generally provide better resolution compared gel-based systems, as well having better sensitivity.
Capillary blood sampling can be used to test for blood glucose (such as in blood glucose monitoring), hemoglobin, pH and lactate. [ 30 ] [ 31 ] It is generally performed by creating a small cut using a blood lancet , followed by sampling by capillary action on the cut with a test strip or small pipette . [ 32 ]
Serum protein electrophoresis (SPEP or SPE) is a laboratory test that examines specific proteins in the blood called globulins. [1] The most common indications for a serum protein electrophoresis test are to diagnose or monitor multiple myeloma , a monoclonal gammopathy of uncertain significance (MGUS), or further investigate a discrepancy ...