Search results
Results from the WOW.Com Content Network
Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g., a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. An inverted microscope is also used for visualisation of the Mycobacterium tuberculosis bacteria in the ...
To keep cells alive during observation, the microscopes are commonly enclosed in a micro cell incubator (the transparent box). Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging ...
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
In this example, the labeled protein has the same abundance in both samples (ratio 1). Stable isotope labeling by/with amino acids in cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling.
The use of oblique (from the side) illumination gives the image a three-dimensional appearance and can highlight otherwise invisible features. A more recent technique based on this method is Hoffmann's modulation contrast, a system found on inverted microscopes for use in cell culture. Oblique illumination enhances contrast even in clear ...
Diagram illustrating the light path through a dark-field microscope. The steps are illustrated in the figure where an inverted microscope is used. Light enters the microscope for illumination of the sample. A specially sized disc, the patch stop (see figure), blocks some light from the light source, leaving an outer ring of illumination. A wide ...
Components of a typical plant cell: a. Plasmodesmata b. Plasma membrane c. Cell wall 1. Chloroplast d. Thylakoid membrane e. Starch grain 2. Vacuole f. Vacuole g. Tonoplast h. Mitochondrion i. Peroxisome j. Cytoplasm k. Small membranous vesicles l. Rough endoplasmic reticulum 3. Nucleus m. Nuclear pore n. Nuclear envelope o. Nucleolus p ...
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.