Search results
Results from the WOW.Com Content Network
In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation, radiation, or heat. [3]
The translocon channel provides a highly heterogeneous environment for the nascent transmembrane α-helices. A relatively polar amphiphilic α-helix can adopt a transmembrane orientation in the translocon (although it would be at the membrane surface or unfolded in vitro ), because its polar residues can face the central water-filled channel of ...
Some examples for applications: Proteinase K is very useful in the isolation of highly native, undamaged DNAs or RNAs, since most microbial or mammalian DNases and RNases are rapidly inactivated by the enzyme, particularly in the presence of 0.5–1% SDS. The enzyme's activity towards native proteins is stimulated by denaturants such as SDS.
A chaotropic agent is a molecule in water solution that can disrupt the hydrogen bonding network between water molecules (i.e. exerts chaotropic activity). This has an effect on the stability of the native state of other molecules in the solution, mainly macromolecules ( proteins , nucleic acids ) by weakening the hydrophobic effect .
This is an accepted version of this page This is the latest accepted revision, reviewed on 2 March 2025. For satirical news, see List of satirical news websites. This is a dynamic list and may never be able to satisfy particular standards for completeness. You can help by adding missing items with reliable sources. Fake news websites are those which intentionally, but not necessarily solely ...
A six-hour manhunt ensued resulting in a police shootout in south Houston in a commercial area off of Stella Link Road. The perpetrator was found hiding in a Dumpster by U.S. Marshal K-9 Rocky.
After going over the few available restricted free agents left, the guys dive deep into the Houston Rockets, who have quickly become one of this offseason’s most interesting teams.
It may be the most widely used lysis buffer. The solubilizing agent is NP-40, which can be replaced by other detergents at different concentrations. Since NP-40 is a nonionic detergent, this lysis buffer has a milder effect than RIPA buffer. It can be used when protein functions are to be retained with minimal disruption. [9] Recipe: [9] 150 mM ...