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  2. RNA-Seq - Wikipedia

    en.wikipedia.org/wiki/RNA-Seq

    Gene length: Longer genes will have more fragments/reads/counts than shorter genes if transcript expression is the same. This is adjusted by dividing the FPM by the length of a feature (which can be a gene, transcript, or exon), resulting in the metric fragments per kilobase of feature per million mapped reads (FPKM). [90]

  3. Read (biology) - Wikipedia

    en.wikipedia.org/wiki/Read_(biology)

    Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]

  4. Massive parallel sequencing - Wikipedia

    en.wikipedia.org/wiki/Massive_parallel_sequencing

    While single-read accuracy is 87%, consensus accuracy has been demonstrated at 99.999% with multi-kilobase read lengths. [ 37 ] [ 38 ] In 2015, Pacific Biosciences released a new sequencing instrument called the Sequel System, which increases capacity approximately 6.5-fold.

  5. Comoving and proper distances - Wikipedia

    en.wikipedia.org/wiki/Comoving_and_proper_distances

    Most large lumps of matter, such as galaxies, are nearly comoving, so that their peculiar velocities (owing to gravitational attraction) are small compared to their Hubble-flow velocity seen by observers in moderately nearby galaxies, (i.e. as seen from galaxies just outside the group local to the observed "lump of matter").

  6. Gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Gel_electrophoresis

    "Most agarose gels are made with between 0.7% (good separation or resolution of large 5–10kb DNA fragments) and 2% (good resolution for small 0.2–1kb fragments) agarose dissolved in electrophoresis buffer. Up to 3% can be used for separating very tiny fragments but a vertical polyacrylamide gel is more appropriate in this case.

  7. Genome size - Wikipedia

    en.wikipedia.org/wiki/Genome_size

    Genome size ranges (in base pairs) of various life forms. Genome size is the total amount of DNA contained within one copy of a single complete genome.It is typically measured in terms of mass in picograms (trillionths or 10 −12 of a gram, abbreviated pg) or less frequently in daltons, or as the total number of nucleotide base pairs, usually in megabases (millions of base pairs, abbreviated ...

  8. Bacterial genome - Wikipedia

    en.wikipedia.org/wiki/Bacterial_genome

    Log-log plot of the total number of annotated proteins in genomes submitted to GenBank as a function of genome size. Based on data from NCBI genome reports.. Bacteria possess a compact genome architecture distinct from eukaryotes in two important ways: bacteria show a strong correlation between genome size and number of functional genes in a genome, and those genes are structured into operons.

  9. Kessler syndrome - Wikipedia

    en.wikipedia.org/wiki/Kessler_syndrome

    One technology proposed to help deal with fragments from 1 to 10 cm (1 ⁄ 2 to 4 in) in size is the laser broom, a proposed multimegawatt land-based laser that could deorbit debris: the side of the debris hit by the laser would ablate and create a thrust that would change the eccentricity of the remains of the fragment until it would re-enter ...