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Nocardia as found on a brain biopsy. Nocardia is a genus of weakly staining Gram-positive, catalase-positive, rod-shaped bacteria. It forms partially acid-fast beaded branching filaments (appearing similar to fungi, but being truly bacteria). It contains a total of 85 species.
The Nocardiaceae form a monophyletic clade within the Corynebacteriales in both 16S rRNA and protein-based phylogenetic trees. [7] [8] A number of conserved signature indels and conserved signature proteins have been identified which are uniquely found in the genera Nocardia and Rhodococcus, supporting a close relationship between the two genera. [8]
Actinomyces is a genus of the Actinomycetia class of bacteria. They all are Gram-positive and facultatively anaerobic , growing best under anaerobic conditions. [ 2 ] Actinomyces species may form endospores , and while individual bacteria are rod-shaped , Actinomyces colonies form fungus -like branched networks of hyphae . [ 3 ]
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Rhodococcus equi is a Gram-positive coccobacillus bacterium. The organism is commonly found in dry and dusty soil and can be important for diseases of domesticated animals (horses and goats). The frequency of infection can reach near 60%. [1] R. equi is an important pathogen causing pneumonia in foals.
A Gram stain of mixed Staphylococcus aureus (S. aureus ATCC 25923, gram-positive cocci, in purple) and Escherichia coli (E. coli ATCC 11775, gram-negative bacilli, in red), the most common Gram stain reference bacteria. Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups ...
Another well publicized find is that of Nocardia as part of the oral microflora. Nocardia spp. have been reported in the normal gingivae and periodontal pockets along with other species such as Actinomyces, Arthromyces [clarification needed] and Streptomyces spp. [8] The usual mode of transmission is inhalation of organisms suspended in dust.
These acids resist staining by ordinary methods such as a Gram stain. [9] It can also be used to stain a few other bacteria, such as Nocardia. The reagents used for Ziehl–Neelsen staining are carbol fuchsin, acid alcohol, and methylene blue. Acid-fast bacilli are bright red after staining. [citation needed]