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The second method of histology processing is called frozen section processing. This is a highly technical scientific method performed by a trained histoscientist. In this method, the tissue is frozen and sliced thinly using a microtome mounted in a below-freezing refrigeration device called the cryostat. The thin frozen sections are mounted on ...
The usual histology slice is cut at 5 to 10 micrometres. The surgical specimen is placed on a metal tissue disc which is then secured in a chuck and frozen rapidly to about –20 to –30 °C. The specimen is placed in a gel-like embedding medium, usually OCT which consists of polyethylene glycol and polyvinyl alcohol ; this compound is known ...
Block holders (in histopathology) used to hold the tissue blocks during cutting •Refrigerated microtome -do-; a special type that is used during operations to aid the surgeon in demarcating the diseased (specially neoplasms) tissue. •Rocking microtome-do-; a special type •Base sledge microtome-do-; a special type •Ultra microtome
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
Pictogram of margin controlled histology or CCPDMA Pictogram of standard bread loafing histology False negative in standard bread loafing histology. Bread loafing is a common method of processing surgical specimens for histopathology. The process involves cutting the specimen into 3 or more sections. [1]
Gross examination of a kidney (right of image) with a renal oncocytoma (left of image).. Gross processing, "grossing" or "gross pathology" is the process by which pathology specimens undergo examination with the bare eye to obtain diagnostic information, as well as cutting and tissue sampling in order to prepare material for subsequent microscopic examination.
The sample of tissue is immersed in fixative solution for a set period of time. The fixative solution must have a volume at least 10 times greater than the volume of the tissue. [6] In order for fixation to be successful, the fixative must diffuse throughout the entire tissue, so tissue size and density, as well as type of fixative must be ...
Automated tissue imaging analysis can significantly reduce uncertainty in characterizing tumors compared to evaluations done by histologists, [1] or improve the prediction rate of recurrence of some cancers. [2] [3] As it is a digital system, suitable for networking, it also facilitates cooperative efforts between distant sites. [4]