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  2. Histopathology - Wikipedia

    en.wikipedia.org/wiki/Histopathology

    The second method of histology processing is called frozen section processing. This is a highly technical scientific method performed by a trained histoscientist. In this method, the tissue is frozen and sliced thinly using a microtome mounted in a below-freezing refrigeration device called the cryostat. The thin frozen sections are mounted on ...

  3. Histology - Wikipedia

    en.wikipedia.org/wiki/Histology

    Similar to the frozen section procedure employed in medicine, cryosectioning is a method to rapidly freeze, cut, and mount sections of tissue for histology. The tissue is usually sectioned on a cryostat or freezing microtome. [12] The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues.

  4. Frozen section procedure - Wikipedia

    en.wikipedia.org/wiki/Frozen_section_procedure

    The usual histology slice is cut at 5 to 10 micrometres. The surgical specimen is placed on a metal tissue disc which is then secured in a chuck and frozen rapidly to about –20 to –30 °C. The specimen is placed in a gel-like embedding medium, usually OCT which consists of polyethylene glycol and polyvinyl alcohol ; this compound is known ...

  5. Instruments used in pathology - Wikipedia

    en.wikipedia.org/wiki/Instruments_used_in_pathology

    Block holders (in histopathology) used to hold the tissue blocks during cutting •Refrigerated microtome -do-; a special type that is used during operations to aid the surgeon in demarcating the diseased (specially neoplasms) tissue. •Rocking microtome-do-; a special type •Base sledge microtome-do-; a special type •Ultra microtome

  6. Automated tissue image analysis - Wikipedia

    en.wikipedia.org/wiki/Automated_tissue_image...

    Microscopic view of a histologic specimen of human lung tissue stained with hematoxylin and eosin. Automated tissue image analysis or histopathology image analysis ( HIMA ) is a process by which computer-controlled automatic test equipment is used to evaluate tissue samples, using computations to derive quantitative measurements from an image ...

  7. Microtome - Wikipedia

    en.wikipedia.org/wiki/Microtome

    From there the tissue can be mounted on a microscope slide, stained with appropriate aqueous dye(s) after removal of the paraffin, and examined using a light microscope. [ 12 ] Frozen section procedure : water-rich tissues are hardened by freezing and cut in the frozen state with a freezing microtome or microtome- cryostat ; sections are ...

  8. Gross processing - Wikipedia

    en.wikipedia.org/wiki/Gross_processing

    Gross examination of a kidney (right of image) with a renal oncocytoma (left of image).. Gross processing, "grossing" or "gross pathology" is the process by which pathology specimens undergo examination with the bare eye to obtain diagnostic information, as well as cutting and tissue sampling in order to prepare material for subsequent microscopic examination.

  9. Microtechnique - Wikipedia

    en.wikipedia.org/wiki/Microtechnique

    The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer

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