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Protein crystallization is the process of formation of a regular array of individual protein molecules stabilized by crystal contacts. If the crystal is sufficiently ordered, it will diffract . Some proteins naturally form crystalline arrays, like aquaporin in the lens of the eye.
Unwanted proteins can be removed from a protein solution mixture by salting out as long as the solubility of the protein in various concentrations of salt solution is known. After removing the precipitate by filtration or centrifugation , the desired protein can be precipitated by altering the salt concentration to the level at which the ...
For protein crystals this method is conducted by soaking the crystal of a sample to be analyzed with a heavy atom solution or co-crystallization with the heavy atom. The addition of the heavy atom (or ion) to the structure should not affect the crystal formation or unit cell dimensions in comparison to its native form, hence, they should be ...
Streak seeding [1] is a method first described during ICCBM-3 by Enrico Stura to induce crystallization in a straight line into a sitting or hanging drop for protein crystallization by introducing microseeds. The purpose is to control nucleation and understand the parameters that make crystals grow. It is also used to test any particular set of ...
X-ray crystallography is the primary method for determining the molecular conformations of biological macromolecules, particularly protein and nucleic acids such as DNA and RNA. The double-helical structure of DNA was deduced from crystallographic data.
Crystallization is the process by which solids form, where the atoms or molecules are highly organized into a structure known as a crystal. Some ways by which crystals form are precipitating from a solution, freezing, or more rarely deposition directly from a gas.
Lipid cubic phases, spontaneous self-assembling liquid crystals or lipid mesophases have been used successfully in the crystallization of integral membrane proteins. [6] Temperature, salts, detergents, various additives are used in this system to tailor the cubic phase to suit the target protein.
Biocrystallization is the formation of crystals from organic macromolecules by living organisms. [1] This may be a stress response, a normal part of metabolism such as processes that dispose of waste compounds, or a pathology. Template mediated crystallization is qualitatively different from in vitro crystallization.